HSP90AA1 monoclonal antibody, clone 4F10
产品名称： HSP90AA1 monoclonal antibody, clone 4F10
英文名称： HSP90AA1 monoclonal antibody, clone 4F10
产品编号： MAB1092
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against full length recombinant HSP90AA1.
- Immunogen:
- Recombinant protein corresponding to full length human HSP90AA1.
- Host:
- Mouse
- Reactivity:
- Human
- Form:
- Liquid
- Isotype:
- IgG2b, kappa
- Storage Buffer:
- In PBS, pH 7.4 (0.1% sodium azide)
- Storage Instruction:
- Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Increased proliferation of B cells and auto-immunity in mice lacking protein kinase Cdelta.
Miyamoto A, Nakayama K, Imaki H, Hirose S, Jiang Y, Abe M, Tsukiyama T, Nagahama H, Ohno S, Hatakeyama S, Nakayama KI.Nature. 2002 Apr 25;416(6883):865-9.
- 2.
- Heat shock protein 90 mediates the balance of nitric oxide and superoxide anion from endothelial nitric-oxide synthase.
Pritchard KA Jr, Ackerman AW, Gross ER, Stepp DW, Shi Y, Fontana JT, Baker JE, Sessa WC.J Biol Chem. 2001 May 25;276(21):17621-4. Epub 2001 Mar 16.
- 3.
- Quantitation and intracellular localization of the 85K heat shock protein by using monoclonal and polyclonal antibodies.
Lai BT, Chin NW, Stanek AE, Keh W, Lanks KW.Mol Cell Biol. 1984 Dec;4(12):2802-10.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of cell lysates of HeLa (each 20 ug) was resolved by SDS - PAGE , transferred to PVDF membrane and probed with HSP90AA1 monoclonal antibody , clone 4F10 (1 : 1000) (Cat # MAB1092). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Paraffin embedded sections of human urothelium were incubated with HSP90AA1 monoclonal antibody, clone 4F10 (Cat # MAB1092) (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
- Entrez GeneID:
- 3320
- GeneBank Accession#:
- NM_005348
- Protein Accession#:
- NP_005339
- Gene Name:
- HSP90AA1
- Gene Alias:
- FLJ31884,HSP86,HSP89A,HSP90A,HSP90N,HSPC1,HSPCA,HSPCAL1,HSPCAL4,HSPN,Hsp89,Hsp90,LAP2
- Gene Description:
- heat shock protein 90kDa alpha (cytosolic), class A member 1
- Omim ID:
- 140571
- Gene Ontology:
- Hyperlink
- Gene Summary:
- HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 (MIM 140572), a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM
- Other Designations:
- heat shock 90kD protein 1, alpha,heat shock 90kD protein 1, alpha-like 4,heat shock 90kD protein, alpha-like 4,heat shock 90kDa protein 1, alpha
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