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Mouse ELA2 / Elane / Elastase-2 Protein
 
 
 
ELA2 / Elane / Elastase-2 Protein Price Inquiry ( Available Sizes )
 
 
 
50μg:  询价;
 
200μg:  询价;
 
≥1mg 大包装:  询价
 
 
 
ELA2 / Elane / Elastase-2蛋白产品信息
 
 
 
 
别名:
 
Elane,  Ela2,   F430011M15Rik,   NE
 
 
 
表达方式:
 
A DNA sequence encoding the mouse ELA2 (NP_056594.2) (Met 1-Arg 260) was fused with a polyhistidine tag at the C-terminus
 
 
 
种属:
 
Mouse
 
 
 
表达宿主: 
 
Human Cells
 
 
 
 
ELA2 / Elane / Elastase-2蛋白质控指标
 
 
 
 
纯度:
 
> 97 % as determined by SDS-PAGE
 
SDS-PAGE:
 
 
ELA2 protein
 
 
 
 
内毒素:
 
< 1.0 EU per μg of the protein as determined by the LAL method.
 
 
 
稳定性:
 
Samples are stable for up to twelve months from date of receipt at -70℃
 
 
 
预测N端:
 
Ser 27
 
 
 
分子量:
 
 
The recombinant mouse ELA2 consists of 245 amino acids with the predicted molecular mass of 27 kDa. As a result of glycosylation, the apparent molecular mass of rm ELA2 is approximately 32 kDa in SDS-PAGE under reducing conditions
 
 
 
 
缓冲液:
 
 
Lyophilized from sterile PBS , pH 7.4
 
 
 
 
 
ELA2 / Elane / Elastase-2蛋白使用指南
 
 
 
 
储存方法:
 
Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
 
 
 
 
 
Reconstitution:
 
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
 
 
 
 
ELA2 / Elane / Elastase-2蛋白相关产品和研究主题
 
 
相关研究领域:
 
Enzyme>>Protease & Regulator>>Serine Protease & Regulator>>ELA2/Neutrophilic Elastase
 
Immunology>>Innate Immunity>>Granulocyte>>Neutrophil granulocyte>>ELA2/Neutrophilic Elastase
 
蛋白:
 
抗体:
 
 
ELA2 / Elane / Elastase-2蛋白背景综述
 
 
Mouse Neutrophil elastase, also known as Elastase-2, Bone marrow serine protease, Medullasin, ELANE, and ELA2, is a serine proteinase in the same family as chymotrypsin and has broad substrate specificity. Secreted by neutrophils during inflammation, it destroys bacteria and host tissue. As with other serine proteinases, ELANE / ELA2 contains a charge relay system composed of the catalytic triad of histidine, aspartate, and serine residues that are dispersed throughout the primary sequence of the polypeptide but that are brought together in the three dimension conformation of the folded protein.
 
ELANE / ELA2 is an important protease enzyme that when expressed aberrantly can cause emphysema or emphysematous changes. This involves breakdown of the lung structure and increased airspaces. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. ELANE / ELA2 hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. ELANE / ELA2 may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. ELANE / ELA2 degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Defects in ELANE are a cause of cyclic haematopoiesis (CH), also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Defects in ELANE are also the cause of autosomal dominant severe congenital neutropenia type 1 (SCN1) which is a heterogeneous disorder of hematopoiesis.
 
 
参考文献
 
 
 
Takahashi H, et al., 1988, J. Biol. Chem. 263 (29): 14739-47.
 
Belaaouaj A, et al., 2000, Science. 289 (5482): 1185-8.
 
Weinrauch Y, et al., 2002, Nature 417 (6884): 91-4.
 
Dale DC, et al., 2009, N. Engl. J. Med. 360 (1): 3-5.
 
 
 
 
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