WNT1 polyclonal antibody
产品名称： WNT1 polyclonal antibody
英文名称： WNT1 polyclonal antibody
产品编号： PAB11291
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of WNT1.
- Immunogen:
- A synthetic peptide corresponding to internal region of human WNT1.
- Host:
- Rabbit
- Reactivity:
- Bovine, Chicken, Dog, Human, Macaque, Mouse, Opossum, Rat
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:25000)
Western Blot (1:1500-1:6000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- The ups and downs of Wnt signaling in prevalent neurological disorders.
De Ferrari GV, Moon RT.Oncogene. 2006 Dec 4;25(57):7545-53.
- 2.
- A Wnt-Axin2-GSK3beta cascade regulates Snail1 activity in breast cancer cells.
Yook JI, Li XY, Ota I, Hu C, Kim HS, Kim NH, Cha SY, Ryu JK, Choi YJ, Kim J, Fearon ER, Weiss SJ.Nat Cell Biol. 2006 Dec;8(12):1398-406. Epub 2006 Oct 29.
- 3.
- Suppressing Wnt signaling by the hedgehog pathway through sFRP-1.
He J, Sheng T, Stelter AA, Li C, Zhang X, Sinha M, Luxon BA, Xie J.J Biol Chem. 2006 Nov 24;281(47):35598-602. Epub 2006 Oct 10.
- Applications
- Western Blot (Cell lysate)
- Western blot using WNT1 polyclonal antibody (Cat # PAB11291) shows detection of endogenous WNT1 in human-derived MCF-7 cell lysate (lane 1) and mouse-derived 3T3 cell lysate (lane 2).
The band at ~41 kDa, indicated by the arrowhead, corresponds to WNT1.
After transfer, the membrane was blocked with 5% BLOTTO.
Primary antibody was used at a 1 : 1,400 dilution in PBS containing 1% BLOTTO.
The specificity of the antibody was confirmed by peptide competition which completely blocked reaction of the antibody with WNT1 (data not shown).
- Entrez GeneID:
- 7471
- Protein Accession#:
- NP_005421.1;P04628
- Gene Name:
- WNT1
- Gene Alias:
- INT1
- Gene Description:
- wingless-type MMTV integration site family, member 1
- Omim ID:
- 164820
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region. [provided by RefSeq
- Other Designations:
- Wingless-type MMTV integration site family, member 1 (oncogene INT1)
- Related Disease